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Bmp4 expression during tooth development of Xenopus laevis
È«Áö¼ö, °íº´¼®, Á¤¾ÆÀ§, À¯¾ç, ¹Úº´°Ç, ÀÌ¿µÈÆ,
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È«Áö¼ö ( Hong Ji-Soo ) - ÀüºÏ´ëÇб³ Ä¡°ú´ëÇÐ ±¸°ÇغÎÇб³½Ç
°íº´¼® ( Ko Byung-Seok ) - ÀüºÏ´ëÇб³ Ä¡°ú´ëÇÐ ±¸°ÇغÎÇб³½Ç
Á¤¾ÆÀ§ ( Cheng Yawei ) - ÀüºÏ´ëÇб³ Ä¡°ú´ëÇÐ ±¸°ÇغÎÇб³½Ç
À¯¾ç ( Liu Yang ) - ÀüºÏ´ëÇб³ Ä¡°ú´ëÇÐ ±¸°ÇغÎÇб³½Ç
¹Úº´°Ç ( Park Byung-Keon ) - ÀüºÏ´ëÇб³ Ä¡°ú´ëÇÐ ±¸°ÇغÎÇб³½Ç
ÀÌ¿µÈÆ ( Lee Young-Hoon ) - ÀüºÏ´ëÇб³ Ä¡°ú´ëÇÐ ±¸°ÇغÎÇб³½Ç
KMID : 0355820190400010019
Abstract
Bmp4´Â Ä¡¾Æ¹ß»ý °úÁ¤¿¡¼ ¹ßÇöÀ» ³ªÅ¸³»°í Ä¡¾ÆÇü¼º À§Ä¡, Ä¡¾Æ¹ß»ý°³½Ã, Ä¡¾ÆÇüÅÂÀÇ Á¶Àý, Ä¡¾Æ¸ð¾çÀÇ °áÁ¤¿¡ °ü¿©ÇÑ´Ù. ±×·¯³ª ¾ÆÇÁ¸®Ä«»ê ¹ßÅé°³±¸¸®ÀÇ Ä¡¾Æ¹ß»ý°úÁ¤¿¡¼´Â Àß ¾Ë·ÁÁ® ÀÖÁö ¾Ê´Ù. ¾ÆÇÁ¸®Ä«»ê ¹ßÅé°³±¸¸®ÀÇ ¾ËÀ» ¼öÁ¤ÇÏ¿© »çÀ°ÇÏ¿© 26ÀÏ°(stage 54)ÀÇ ¿ÃìÀ̺ÎÅÍ º¯Å Á÷ÈÄ ¾î¸° °³±¸¸®ÀÇ À§ÅÎÀ» in situ hybridization ¹æ¹ýÀ¸·Î ½ÇÇèÇÏ¿´´Ù. Bmp4´Â stage 54ÀÇ Ä¡¹è¿¡¼´Â ¹ßÇöµÇÁö ¾Ê¾Ò°í, stage 56ºÎÅÍ stage 62±îÁö Áß°£¿±°ú Ä¡¾ÆÀ¯µÎ, ¹Ù±ùÄ¡¾Æ»óÇÇ¿¡¼ ¹ßÇöµÇ¾ú´Ù. º¯Å Á÷ÈÄ ¾î¸° °³±¸¸®¿¡ ÇØ´çÇÏ´Â stage 66¿¡¼, Bmp4 ´Â À§ÅλÀ¿¡ À¯ÂøµÈ Ä¡¾Æ¿¡¼ ¹ßÇöµÇÁö ¾Ê¾Ò°í, °è½ÂÄ¡¾ÆÀÇ Áß°£¿±°ú ¹Ù±ùÄ¡¾Æ»óÇÇ¿¡¼ ¹ßÇöµÇ¾ú´Ù. ±×·¯¹Ç·Î Bmp4´Â ¾ÆÇÁ¸®Ä«»ê ¹ßÅé°³±¸¸®ÀÇ Ä¡¾Æ¹ß»ý °úÁ¤¿¡¼ »ó¾ÆÁú Çü¼º¿¡ Áß¿äÇÑ ¿ªÇÒÀ» ÇÒ °ÍÀ¸·Î »ý°¢µÈ´Ù.
Bmp4 exhibits expression patterns throughout tooth morphogenesis and is involved in positioning the sites of tooth formation, initiating tooth development, regulating tooth morphogenesis, determining tooth shape, and promoting the differentiation of tooth-specific cell types. However, little is known about bmp4 expression during tooth development in Xenopus. Bmp4 expression was examined by in situ hybridization during Xenopus tooth development from 26 days post-fertilization (PF, stage 54) to 58 days PF (stage 66). Bmp4 was not shown to be expressed at stage 54 before tooth germ formation. From stage 56 to 62, bmp4 expression was observed in the dental mesenchyme, dental papilla, and outer enamel epithelium. Bmp4 was expressed strongly in the dental mesenchyme and weakly in the outer enamel epithelium. At stage 66 (58 days PF), bmp4 expression was not detected in the tooth ankylosed to the maxillary bone; however, the transcripts showed an expression pattern similar to the previous stage in the successive tooth germ. These results suggest that bmp4 may play a critical role in dentin formation during tooth development of Xenopus laevis.
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Xenopus laevis; tooth development; Bmp4 expression
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